A Comparative Study of <i>Helicobacter pylori</i> Growth on Different Agar-based Media

Lee, Jung Hwan; Park, Jiwan; Park, Mi Ri; Na, Yoon Hee; Cho, Soo Jeong

doi:2017.17.4.208

Korean J Helicobacter Up Gastrointest Res > Volume 17(4); 2017 > Article

Original Article

The Korean Journal of Helicobacter and Upper Gastrointestinal Research 2017;17(4):208-212.
DOI: https://doi.org/10.7704/kjhugr.2017.17.4.208 Published online December 10, 2017.

A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media

Jung Hwan Lee, Jiwan Park, Mi Ri Park, Yoon Hee Na, Soo Jeong Cho

Center for Gastric Cancer, National Cancer Center, Goyang, Korea. crystal522@daum.net

Received: 22 May 2017 • Revised: 3 July 2017 • Accepted: 4 July 2017

Abstract

BACKGROUND/AIMS
Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori. MATERIALS AND METHODS: G27, ATCC #43504 and 60190, and primary cultured strains were used. H. pylori strains were cultured for four days under four culture conditions: chocolate agar, Thayer-Martin (TM) agar containing vancomycin-colistin-nystatin inhibitor (VCNI), Brucella agar, and brain heart infusion (BHI) agar containing 5% horse blood and IsoVitaleX (BBL™ BD, USA). Culture of cells in each medium was repeated fourteen times. The growth of H. pylori was measured by using a spectrophotometer. RESULTS: TM, Brucella, and BHI agars showed mean absorbance values of 0.099, 0.059, 1.410, and 0.913, respectively. These values were significantly different (P=0.030). After post-adjustment by Bonferroni correction, similar growth was noted for in chocolate, Brucella, and BHI agars; however, TM agar significantly suppressed H. pylori growth compared with Brucella agar (P=0.031). CONCLUSIONS: Chocolate, Brucella, and BHI agars provided effective culture conditions for the growth of H. pylori. TM agar containing VCNI suppressed the growth of H. pylori and other organisms.

Key Words: Agar; Culture; Helicobacter pylori; Media