Analysis of Antimicrobial Resistance of <i>Helicobacter pylori</i> in Gwangju, Chonnam Provinces

Kim, Min Hyung; Kim, Hyun Soo; Park, Sun Young; Park, Chang Hwan; Choi, Sung Kyu; Rew, Jong Sun

doi:2012.12.2.82

Korean J Helicobacter Up Gastrointest Res > Volume 12(2); 2012 > Article

Original Article

The Korean Journal of Helicobacter and Upper Gastrointestinal Research 2012;12(2):82-87.
DOI: https://doi.org/10.7704/kjhugr.2012.12.2.82 Published online June 1, 2012.

Analysis of Antimicrobial Resistance of Helicobacter pylori in Gwangju, Chonnam Provinces

Min Hyung Kim, Hyun Soo Kim, Sun Young Park, Chang Hwan Park, Sung Kyu Choi, Jong Sun Rew

Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea. dshskim@jnu.ac.kr

광주 전남지역에서 Helicobacter pylori 제균 항생제 내성률 분석

김민형, 김현수, 박선영, 박창환, 최성규, 유종선

전남대학교 의과대학 내과학교실

Abstract

BACKGROUND/AIMS
A triple therapy, comprised of a proton pump inhibitor, clarithromycin, and amoxicillin or metronidazole, is the most commonly used treatment for Helicobacter pylori (H. pylori) eradication. But, H. pylori have become resistant to antimicrobial agents, reducing eradication rates. Thus, we investigated the distribution of minimal inhibitory concentration (MIC) for these drugs and analyzed the factors that affect it. MATERIALS AND METHODS: From July 2008 through December 2009, we performed tissue culture with gastric mucosa from 51 patients who were infected with H. pylori and measured MIC by Epsilometer test (E-test). RESULTS: The mean age of the group was 56.8+/-13.8 years, and the male to female ratio was 2:1. Resistance rates of H. pylori to amoxillin, clarithromycin, metronidazole, ciprofloxacin, levofloxacin, tetracycline were 2%, 15.7%, 21.6%, 25.5%, 25.5%, and 0%, respectively. Statistically, there were no significant relationship between resistance rate and other factors including age, sex, alcohol or smoking. Follow up test revealed eradication failure in 4 of 22 patients but, MIC for amoxillin and clarithromycin of these four patients were considered to belong to the susceptible group. Three patients belonging to the clarithromycin-resistant group (MIC>256 microg/mL), were eradicated. In addition, one of the three patients showed resistant to not only clarithromycin (MIC 12 microg/mL), but also amoxiciliin (MIC 0.6 microg/mL). CONCLUSIONS: The microbial susceptability test of each antibiotics does not accurately reflecte successful eradication, but further investigation is needed to identify the factors that influence the eradication of H. pylori.

Key Words: Helicobacter pylori; Micribial sensitivity test